Retraction: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10
نویسندگان
چکیده
Retraction The authors have retracted this article [1] due to the ethical misconduct of Karel Bezouska and misappropriation of data which fundamentally changes the main findings of the article. Specifically, the upper amino acid sequence in Figure 1 (by KB) in BMC Biotechnol 11:2 was incorrect, therefore Nit-ANigWT and Nit-ANigRec (nitrilase expressed in E. coli harbouring a nit gene from A. niger K10) were not variants of the same enzyme as hypothesized in the original article. The different biochemical properties of Nit-ANigWT and Nit-ANigRec discussed in the paper were caused by a significant difference in the primary structures of these enzymes.
منابع مشابه
Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10
BACKGROUND Nitrilases attract increasing attention due to their utility in the mild hydrolysis of nitriles. According to activity and gene screening, filamentous fungi are a rich source of nitrilases distinct in evolution from their widely examined bacterial counterparts. However, fungal nitrilases have been less explored than the bacterial ones. Nitrilases are typically heterogeneous in their ...
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